OSU-T315 (15-regioisomer)

Research use only, not for human use.

OSU-T315 (15-regioisomer) is a ILK inhibitor.

OSU-T315 (15-regioisomer) is a ILK inhibitor.

OSU-T315 (Compound 22; 0-5 μM; 24 hours) exhibits high in vitro potency against a panel of prostate and breast cancer cell lines with a IC50 range of 1-2.5 μM.?OSU-T315 (0-2.5 μM; 24 hours) can reduce YB-1, HER2, and EGFR expression; shows a modest suppressive effect on phosphorylated S6 levels, exhibits dose-dependent suppressive effects on the levels of phospho-ERK1/2 and phospho-p38, while that of phospho-JNK remains unaltered in PC-3 cell. OSU-T315 (0-4 μM; 24 hours) causes autophagy through ILK inhibition. Western Blot Analysis Cell Line:PC-3 cells; MDA-MB-231 cells Concentration:1 μM, 2 μM, 3 μM, 4 μM; 0.5 μM, 1 μM, 1.5 μM, 2 μM, 2.5 μM Incubation Time:24 hours Result:Exhibited a dose-dependent decreasing effect on the phosphorylation of pS6, ERKs, and p38 in PC-3 cells and MDA-MB-231 cells.Cell Viability Assay Cell Line:Prostate cancer cells: LNCaP, PC-3; breast cancer cells: MDA-MB-231, MDA-MB-468, SKBR3, MCF-7; PrEC and MEC cells Concentration:0-5 μM Incubation Time:24 hours Result:Suppressed cancer cells viability in breast and prostate cancer cells (IC (50), 1-2.5μM).Apoptosis Analysis Cell Line:PC-3 cells Concentration:1 μM, 2 μM, 3 μM, 4 μM Incubation Time:24 hours Result:Induced accumulation of LC3-II and PARP cleavage.

OSU-T315 (Oral gavage; 25 mg/kg, 50 mg/kg; single daily; 35 days) has a suppressive effect of on PC-3 xenograft tumor growth . No other obvious toxicity is observed in mice. Animal Model: Male NCr athymic nude mice with PC-3 tumor xenografts Dosage:25 mg/kg; 50 mg/kg Administration:Oral gavage; single daily; 35 days Result:Resulted in suppression of tumor growth relative to the vehicle control after 35 days of treatment (48% and 62% suppression for 25 and 50 mg/kg, respectively).

ILK-IN-2

Cell Cycle/DNA Damage

Integrin

ILK

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2070015-22-2

533.6

C30H30F3N5O

>98% (HPLC)

DMSO:50 mg/mL?(93.70 mM)

CNC(=O)CCc1cc(nn1-c1ccc(cc1)N1CCNCC1)-c1ccc(cc1)-c1ccc(cc1)C(F)(F)F

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(-20℃)

With Ice Pack

≥ 2 years